Dynamics of Mpox infection in Nigeria: a systematic review and meta-analysis

The seasonal outbreaks of Mpox continue in most parts of West and Central Africa. In the past year, Nigeria had the highest number of reported cases. Here, we used the PRISMA guidelines to carry out a systematic review and meta-analysis of available evidence on Mpox in Nigeria to assess the prevalence, transmission pattern, diagnostic approach, and other associated factors useful for mitigating the transmission of the disease. All relevant observational studies in PubMed/MEDLINE, Embase, AJOL, Web of Science, Scopus and Google Scholar on Mpox in Nigeria were assessed within the last fifty years (1972 to 2022). In all, 92 relevant articles were retrieved, out of which 23 were included in the final qualitative analysis. Notably, most of the cases of Mpox in Nigeria were from the southern part of the country. Our findings showed a progressive spread from the southern to the northern region of the country. We identified the following factors as important in the transmission of Mpox in Nigeria; poverty, lack of basic healthcare facilities, and risk of exposure through unsafe sexual practices. Our findings reiterate the need to strengthen and expand existing efforts as well as establish robust multi-sectoral collaboration to understand the dynamics of Mpox Nigeria.

have been suggested as natural hosts of the virus 1,2,20,21 .The Mpox virus is transmitted from animals to humans, mainly through direct contact with blood, body fluids, or cutaneous or mucosal lesions of infected animals 22,23 .Human-to-human transmission has also been reported via direct contact with infected materials from skin lesions of infected persons, through respiratory droplets following prolonged face-to-face contact, and through recently contaminated objects such as cloths and beddings 5,8,22 .Vertical transmission through the placenta and during childbirth has also been reported 22,23 .Additionally, the epidemiological patterns of recent outbreaks in Europe suggested a sexual route of transmission 5,24 .Although the mechanism of sexual transmission has not been fully elucidated 8,[24][25][26] , recent reports have established sexual transmission 25,[27][28][29][30][31][32] .Over 80% of cases were in men who had sex with men 32 .
The clinical symptoms of Mpox infection include fever, headache, muscle pains, general body weakness and lymphadenopathy, which are the main features of the first phase of the disease.The second phase, which manifests with rashes, usually begins 1-3 days after the onset of fever.Mpox infections resolve independently within 2 to 4 weeks in healthy individuals.More severe forms of the disease occur among children and immunocompromised individuals 29 .
Nigeria has the highest reported cases (842) of Mpox in Africa as of 19 May 2023, followed by DRC (739), Ghana (27), Central African Republic (30), and Cameroon (29)  32 .Since the re-emergence of the disease in Nigeria between September 2017 and August 7, 2022, a total of 985 suspected Mpox cases have been reported, with 398 (40.4%) confirmed and 12 deaths (CFR = 3.0%) 33 .Approximately 66.1% of the cases were males.Thirty of the 36 states and the Federal Capital Territory (FCT) have reported at least one case (Table 1) 14,34,35 .Most of the high-burden states in Nigeria are located within the forest belt of the country 34 .This finding is consistent with the known nidation of Mpox transmission in the forested regions of West and Central Africa 3,23,36 .However, most cases reported in Nigeria were in urban centres and cities, probably a reflection of the locations of large hospitals and diagnostic facilities in urban areas rather than an indication of a rural-urban geographical divide in case distribution 14,37 .
It is widely believed that the number of Mpox cases is grossly underestimated since reports of cases which are asymptomatic have recently emerged 29 .In Nigeria, underreporting may result from factors such as a poor index of suspicion due to lack of awareness of healthcare workers, poor surveillance and weak health systems.Other factors include inadequate number of laboratories with the capacity to diagnose the disease, lack of access to approved antiviral medicines and vaccines, and stigmatisation 14,[38][39][40][41] .Nigeria with a population of a median age of 18.6 years, most of whom have never been vaccinated against smallpox, has among the largest pools of individuals susceptible to Mpox in the world 42 .A large proportion of these vulnerable individuals are women and children 43 .The country also ranks among the highest in terms of the burden of HIV/AIDS, tuberculosis, hunger and malnutrition/undernutrition, malaria and insecurity [44][45][46][47] .These are all known risk factors for Mpox and many other infectious diseases 3,29,48,49 .Thus, a national strategy to interrupt local transmission of the disease is urgently needed.So far, the spread of the disease from Nigeria to Israel (2018), the United Kingdom (September 2018, December 2019, May 2021 and May 2022), Singapore (May 2019) and the United States (July and November 2021) have been documented 6,29,[50][51][52] .Thus, continued local transmission of Mpox in Nigeria may have implications for global health.This study, therefore, sets out to assess the prevalence and distribution of Mpox cases, transmission patterns, impact of comorbidities and existing diagnostic protocols for Mpox in Nigeria.This is aimed at providing useful baseline information for effective policy development geared toward mitigating the spread of the disease in the country.

Characteristics of studies
A total of 92 articles were retrieved by literature search (79 from a database search and 13 from other sources).Of these, 72 articles were retained after duplicates were removed.Further, 32 were excluded due to irrelevance to the subject matter.The remaining 40 articles and abstracts were further screened based on the inclusion and exclusion criteria, and 17 were excluded.Finally, after a detailed full-text evaluation, 23 articles published between 1972 and 2022 were selected for qualitative analysis using the EPHPP.Only 12 of these articles were selected for quantitative analysis (Fig. 1).Of the 23 studies selected, 14 (60.9%) were case reports, six (26.2%) cross-sectional studies, one (4.3%)purposive cohort study, one case-control study (4.3%), and one (4.3%)retrospective cohort study.Eight (34.8%) of the selected studies were based on Mpox cases exported from Nigeria to other parts of the world.Fourteen (60.9%) of the cases were based solely on reports from the southern part of Nigeria, two (8.7%) were cases from northern Nigeria, and seven (30.4%) were based on cases reported across both the northern and southern parts of the country (Table 2).In addition to the included studies, information from 26 grey literature, obtained from the references of the included studies, were utilized in the review.
Across the six quality domains evaluated, all of the studies met three or more quality criteria, and most of the studies (n = 16) met four to six of the quality criteria assessed.The most common quality criteria failed by the studies were sample size, identification of subgroups, subjects and settings.All publications, however, passed the population under consideration criterion.
Between 2017 and 2018, 17 states in Nigeria reported at least one confirmed case of Mpox (Fig. 2A) 14 .All states in the country's South-south and Southeast geopolitical zone, except for Ebonyi State, reported at least one confirmed case of Mpox.Three states in the southwest (Lagos, Oyo, and Ekiti) and four in the Northcentral geopolitical zone (Plateau, Nasarawa, the Federal Capital Territory, and Benue) also reported at least one confirmed case.Nigeria's Northwest and Northeast geopolitical zones did not report any confirmed cases of Mpox between 2017 and 2018 14 .
According to the NCDC, as of August 28, 2022, thirty-two states in Nigeria had reported at least a confirmed case of Mpox in the country.All the states in the South-South, South-East, South-West, and North-Central
The studies considered for suspected and confirmed cases in Fig. 3A showed a high degree of heterogeneity, I 2 = 85%.There exists a significant difference between the number of confirmed cases and suspected cases, and this was not due to chance, as shown by a statistical significance pooled estimate with a point estimate of 1.63 ).This finding indicates that the majority of the suspected cases were not confirmed either due to diagnostic tools inadequacy or non-specificity of clinical signs (Fig. 3A).The confirmed cases compared to the number of deaths in all the reviewed articles showed homogeneity, I 2 = 0% (Fig. 3B).This finding shows that across all the studies considered, the rate of fatality reported compared to the confirmed cases is consistent regardless of the location.Further, the forest plot that compared the cases of Mpox between males and females showed a significant difference in the number of reported cases between the genders, and this was not due to chance.The reviewed studies also showed a calculated heterogeneity value (I 2 ) of 50%, indicating average differences between studies.This is evident in the statistical significance of the pooled estimate with the point estimate (2.01) confidence interval (1.30-3.10)and a probability value (p = 0.002).This finding indicates a significant difference in cases reported between the two genders, with more cases reported in males (Fig. 3C).

Poverty
Four reported cases of human infection with Mpox linked to individuals most of whom reside in the urban slums/ sub-urban and rural areas were identified 62,66,70,76 .These included two female traders who reside in a rural area of southern Nigeria and presented to healthcare facility with numerous cutaneous eruption (papules, pustules and nodules) 66 .The others involved two cases of 20 a year old man and his step brother, both living in an urban slum in Northcentral Nigeria 62 .The first case, presented with a week's history of fever, headache, pain on swallowing and micturition, and generalised skin lesions.The second, his step brother, and the primary care provider to the first case when he took ill, also presented with 1-week's history of fever, headache, pain on swallowing, and skin lesions which were less intense than in the first case 62 .Notably, six cases of Mpox infection were reported in the rural/urban slums of Liberia, Nigeria and Sierra Leone between 1970-1971 76 , two of which were from Nigeria.

Lack of basic healthcare facilities
Five studies associated with cases of Mpox infection and its severity to inadequate healthcare facilities including diagnosis and patients management 14,59,67,70,71 were reviewed.Enhanced surveillance and strengthening of diagnostic tools/facilities have helped to improve the detection of more cases in real-time, especially in hotspot locations 14,70,71 .With optimal diagnostic facility, a man (the index case) and his family, including his wife travelled from southwestern Nigeria to the UK.The man, his wife and a caregiver were confirmed with Mpox infection following a PCR testing in the UK.As a result of the availability of required healthcare facilities, an in-depth contact tracing and active surveillance ensured that no transmission outside the index family occurred 59 .In contrast to the first outbreak of Mpox reported in Akwa Ibom, south-south of Nigeria, due to inadequate logistics for sample collection, transportation and diagnosis, needed active case detection and surveillance mechanisms could not be carried out.Unfortunately, only few suspected cases were assessed with limited samples collected, and those collected could not be processed due to logistics and diagnostics challenges 67 .

Risk of exposure through unsafe sexual practices
Three studies reported the detection of highest proportion of human Mpox infection among sexually active age groups 67,68,87 .In two studies, more than half (53%) and about half (47%) of the entire study population were reported to be infected with Mpox cases, respectively 67,68 .Specifically, a study made a case for the role of sexual contact in the transmission of Mpox among confirmed cases from Nigeria.In the study, following the survey of sexual history of participants, and other associated risk behaviours and practices, 81.2% had genital ulcers, 56.2% reported to engage in high-risk behaviours (like unprotected sex, multiple sexual partners, and transactional sex) and 50% had sexual intercourse within a month before their first symptoms 87 .

Mode of transmission
Consumption of bush meat was the most prevalent mode of Mpox transmission recorded 56,57,61,67,70 .Other means of transmission included contact with rodent carcasses 56 , occupational risk (nosocomial) 56 , direct respiratory and mucosal contact with an infected person 60,67,71 , and sexual contact 57 .Other papers examined did not record a specific transmission mechanism 58,59 .Generally, various transmission routes have been reported, ranging from the most common animal-to-human transmission (zoonotic), human-to-human (horizontal transmission), human-to-animal (zooanthroponotic transmission), as well as animal-to-animal transmission 72 (Fig. 4).

Effect of concurrent infections/comorbidities
Four of the reviewed articles had information about co-infections.In the case of Mpox co-infection with HIV, a longer duration of disease, bigger lesions, severe cases of genital ulcers, and an increased risk of subsequent bacterial skin infection were observed 14,54,56,73 .In another instance in which a pregnant lady had Mpox, the infection led to premature membrane (amniotic sac) rupture and the subsequent vaginal delivery of a macerated fetus 73 .

Exportation of Mpox from Nigeria to other parts of the world
Between 2018 and 2022, four countries reported the importation of Mpox cases from Nigeria.These are Singapore 56,57 , Israel 55,56 , the United Kingdom 56,59,61 , and the United States 58,60 .

Discussion
A comprehensive study of the reviewed published literature on Mpox in Nigeria revealed several important facts.Nigeria occupies a central position in the current Mpox outbreak because researchers have traced the likely source of the outbreak to the country prior to 2017 14 .This single but important fact is a cause for concern considering the inconsistency and structural deficiency in the healthcare system of the country that has made the execution of preparedness plans, prompt response to disease outbreaks as well as robust contact tracing difficult to achieve.
It should be noted that Mpox infection is on the rise mostly in low-and middle-income countries of Africa due in part to the unavailability of vaccines, lack of information among at-risk people, or fear of seeking information due to the criminalisation of gay sex.Although researchers around the world have posited that Mpox may not cause any massive magnitude of infections, however, the disease has come to stay and eradicating it may be difficult due to constant mutations by the virus 77 .However, according to Tomori and Ogoina, the consequences of neglecting a disease anywhere around the world could be costly because a disease anywhere can spread everywhere if not given the required attention 78 .
This review revealed that despite inadequacies of the disease surveillance system in Nigeria, the cases reported have been on the increase since 2017.In addition, majority of the cases reported seem to be spillovers from animal hosts and not necessarily from outside the country.Thereafter, the infection has continued to spread within the human population.Previous studies have identified spillover hosts and poor disease surveillance as major culprits responsible for the continuous spread of the disease in Nigeria 79,80 .
The majority of Mpox cases in Nigeria were reported in the southern region of the country.This finding is consistent with the previous work that established the connection between Mpox transmission and the rainforest environment 79 .Notably, the availability of better disease surveillance and diagnostic facilities and infrastructure could be responsible for this finding.It is, however, important to note that before the 2005 outbreak in South Sudan, it was believed that Mpox was confined to the rainforest regions 81 .Importantly, the studies also revealed a progressive spread of the disease from the south to the northern part of Nigeria.In 2018, although most states in the southern region had already recorded at least a case of Mpox, only a few northern states had reported cases of the disease.Currently, as at the time of this review in 2023, the majority of states in northern Nigeria, especially in the north-central and northeastern regions, have also recorded at least one case of Mpox 52,82 , indicating a remarkable spread in the cases of Mpox within a short period.
The northern region of Nigeria is mostly semi-desert to desert (i.e., Guinea and Sudan savannah) in terms of vegetation, whereas the southern region is rainforest.Consequently, it is anticipated that the disease will spread www.nature.com/scientificreports/from locations adjacent to the natural habitats of reservoir animals to regions that are far from such habitats.Therefore, it may be hypothesised that the majority of cases in the north are a result of direct or indirect contact with people who have contracted the disease and not due to direct contact with reservoir animals.Similarly, most cases were reported in the rural areas of the country 71 .This finding establishes a connection between poverty and lack of access to basic amenities in Nigeria and the spread of Mpox, as previously reported 83 .
Although the population of individuals affected by Mpox in Nigeria belong to a wide age range, most of the cases were reported among adults 25 years (about 85%) and above.The detection of cases among a few married sexual partners and the high rates of genital ulcers suggest a role of sexual contact in the transmission of MPX during the 2017 outbreak in Nigeria 14 .Further, most cases were reported among young to adult males.This finding indicates that the likelihood of males contracting Mpox in Nigeria is significantly greater than the likelihood of females contracting the disease.
The high incidence of Mpox among sexually active males in Nigeria can be partly explained by the recent surge in male-to-male sexual intimacy among Africans 84 and is consistent with the reported incidence of Mpox among adult males with multiple sexual partners in developed nations 85 .Also, the likelihood of a man contracting the disease during a male-to-male sexual act is greater than the likelihood of disease transmission through a female-to-male sexual encounter, owing to the prolonged shedding of the virus in seminal fluid 7,86,87 .The issue of men who have sex with men (MSM) as a major risk factor for the increase in the spread of Mpox in Nigeria requires further investigation.
Socio-cultural practices play a crucial part in the transmission of Mpox.Many Nigerians consume bush meat, which is sometimes either improperly or unhygienically prepared.Since most of the animals that have been linked to Mpox transmission are associated with the wild, they could serve as reservoir/spillover hosts for the virus 88 .It is therefore not strange that higher incidence of the disease is being reported in the southern part of the country where wild animals are abundant due to the presence of forests, an ideal habitat for these animal reservoirs 82 .The exportation of the disease from Nigeria to other nations, such as the United Kingdom, Singapore, Israel, and the United States, as documented in this review, supports the transboundary nature of the Mpox virus [55][56][57] .
Confirmation of Mpox in Nigeria is based mainly on the use of molecular techniques such as PCR and genome sequencing.However, the equipment required for these procedures are not widely distributed throughout the country but are limited to a few referral laboratories in the urban centres.The use of these molecular methods provides a definitive diagnosis of the Mpox cases in Nigeria, although several cases might remain undiagnosed/ undetected.Other diagnostic methods used include IgM antibody detection, electron microscopy, ELISA, immunofluorescent assay, and agar gel immunodiffusion test.
Overall, not all suspected cases of Mpox were confirmed to be the disease in Nigeria.This demonstrates the existence of other diseases with similar clinical manifestations and symptoms to Mpox in Nigeria.It is, therefore, essential to pay close attention to the occurrence of diseases with symptoms similar to those of Mpox among Nigerians.Some of these diseases include measles, chickenpox, smallpox, and other skin infections that present with skin rashes and related lesions.
Compared to the number of confirmed Mpox cases, the case fatality rate reported in the reviewed articles was low (5.8%).This finding further demonstrates that Mpox is a self-limiting disease, with only immunocompromised individuals experiencing the severe form 89 .The worsening of Mpox symptoms in the presence of co-infection with other immunocompromising diseases like HIV, TB and hepatitis supports the notion that, although the disease is self-limiting, a healthy immune system is essential to limit its progression and halt it within the expected two to four weeks 90 .
The findings of this review further give credence to the fact that immunocompromised individuals are more likely to suffer from the severe form of Mpox.Further, it was observed that contracting Mpox infection during pregnancy can cause the early rupture of the amniotic sac and the subsequent delivery of a mummified fetus.This assertion was further substantiated by studies that revealed the teratogenic effect of pox viruses 91,92 .

Conclusion
There is a huge gap in knowledge on the current Mpox outbreak and control situation in Nigeria.Nigeria plays a key role in the exportation of Mpox to other countries.Hence, understanding the dynamics of the disease will help in solving other emerging/re-emerging infectious disease emergencies in Nigeria and in other countries with similar socio-cultural and ecological settings, as well as prevent future international spread of these diseases.Efforts are ongoing to perform genomics sequencing of all Mpox-positive samples by the NCDC.However, more still needs to be done by the NCDC in the area of coordination of already existing disease diagnostic infrastructures by getting the states more involved in surveillance systems.This will provide information on the source of index, transmission pattern, major drivers of the infection (socioecological factors), and the spread of the infection/disease and supplement efforts by the NCDC.Furthermore, there is still a need for robust multidisciplinary/multi-sectoral collaboration between researchers within and outside the country to find answers to some pertinent questions relating to the animal reservoirs of Mpox.These initiatives will help provide insight into the mode of transmission, presentation (especially viz-a-viz the possibility of asymptomatic carriers) and the need for partnerships to build a robust community-driven surveillance network for early detection, early response and reporting of cases of Mpox and other epidemic-prone diseases.

Methods
A systematic assessment of published studies and reports on human Mpox cases in Nigeria was carried out based on the PRISMA recommendation.We searched for publications in PubMed/MEDLINE, Embase, AJOL, Web of Science, Scopus and Google Scholar for studies (full articles and abstracts) published within 1972 to 2022, involving the prevalence or incidence of human Mpox in Nigeria (and those with links to Nigeria).Key www.nature.com/scientificreports/search words were used without regard to language.We also searched the internet for the study headings, titles, or abstracts (Supplementary Information).

Search strategy
The

Grey literature
All relevant information from government and international organisations' websites and repositories were obtained from the references of the reviewed articles that met our inclusion criteria, and were categorised as grey literature.These materials, though relevant, did not meet our inclusion criteria because they were not articles published in peer reviewed journals, rather they are periodic information in the website of these international/ national organisations/bodies.In all 26 articles were obtained from grey literature comprising eight (8)

Selection strategy
The initial selection by title and abstract was conducted independently by two researchers: MB and TO according to the inclusion/exclusion criteria.All articles that presented one or more terms with Mpox and Nigeria relationship were included.Subsequently, an exhaustive reading of the articles was carried out to confirm inclusion of relevant data for the systematic review and important variables for the meta-analysis.The final decision on articles to be included was discussed with a third investigator (VA), and a consensus was reached.
The PRISMA model was used to organise the information from the article selection process.

Inclusion and exclusion criteria
The final reviewed articles and abstracts included prospective observational studies, case reports (including exported cases, i.e. those that have links/sources linked with Nigeria), cohort studies, and epidemiological investigations that reported on Mpox in humans were included.Resources excluded include full books, book sections, studies describing study populations not based in Nigeria, studies on animals or insects, studies not focused on Mpox patients exclusively, retrospective studies, reviews, editorials and publications lacking original data (transitory website-based information).Likewise, conference abstracts, conference proceedings, and review articles were excluded from the analysis.

Data extraction
Using the inclusion and exclusion criteria, three reviewers (MB, TO and JF) independently screened the titles and abstracts of eligible studies using the Rayyan screening tool 93 .The whole text of the citations chosen for evaluation was obtained, and the reviewers independently collected all study data and resolved disagreements by consensus.The extracted data from each article included the first author, year of publication, study location, study enrolment period, number of suspected cases and the number of confirmed cases.Other information collected included the technique of diagnosis, comorbidity and case fatality rate.Other data obtained included age and gender of study participants/case reported, study participants, sample size and reported incidence/prevalence with 95% confidence intervals (CIs).If available, data on diagnostic protocol, transmission pattern, and comorbidities were included.The three reviewers also performed data extraction and resolved all disagreements by consensus.

Quality assessment and risk of bias
The quality assessment of the study was carried out using the Effective Public Health Practice Project (EPHPP), a quality assessment tool for quantitative studies 94 .This tool ensures the evaluation of the risk of bias rather than excluding low quality literature.The risk of bias in each retrieved document was evaluated based on the population considered, sample size adequacy, study settings, diagnostic tool used, and subgroup reported.

Selection bias
This refers to the individuals who participated in the study under consideration.
Strong/moderate-if the individuals are solely representative of the prevalence or incidence of human Mpox in Nigeria; and more than 60% of the selected individuals agreed to participate in the study.
Weak-if the study is based on individuals from studies of diseases other than Mpox and if neither the research participants nor the occurrence can be traced to Nigeria.

Subject and setting
This pertains to the type of study being evaluated by the article under review.
Strong/moderate-if the article is based on cohort studies, observational studies, epidemiological studies, and case reports conducted on Mpox in Nigeria.
Weak-if the article is a book, book section, or research describing non-Nigerian populations i.e. participants who were believed to have contracted Mpox in Nigeria before going to other nations.

Diagnostic tool deployed
This relates to the dependability of the diagnostic techniques deployed.
Strong/moderate-if molecular techniques were utilised in the diagnosis of Mpox and if more than one diagnostic technique was used.
Weak-if Mpox was diagnosed using non-molecular techniques.

Subgroups identified
Strong/moderate-provided the study acknowledged the presence of distinct subgroups, such as gender and age.
Weak-if the study does not identify subgroups.The studies that were identified as been weak based on the above described criteria were considered to show high level of bias and those that fell within the strong/moderate category were adjudged low and moderately biased.These were the studies included in the qualitative and quantitative analysis.

Suspected cases
We identified suspected cases as those with sudden onset of high fever, followed by a vesicular-pustular eruption showing predominantly on the face, palms of the hands, and soles of the feet or the presence of at least five scabs resembling smallpox lesions.

Confirmed cases
We identified confirmed cases as suspected cases confirmed by laboratory tests or analysis (positive IgM antibody, PCR, or virus isolation).

Probable cases
A suspected case with an epidemiological connection to a confirmed case but no chance for laboratory confirmation.

Possible cases
A case with a vesicular, pustular, or crusted rash that was not identified as chickenpox by the patient's family or physician.
Fever with vesicular or crusty rash prior history.Individuals with unexplained rash, fever, and at least two additional clinically relevant symptoms in addition to meeting one of the epidemiological criteria or exhibiting increased levels of orthopoxvirus-specific IgM.

Statistical analysis
Data were analysed using Revman Review Manager Version 5.4 software.The calculated results were presented in tables and graphs using descriptive statistics.The heterogeneity across studies was evaluated by Cochrane's Q-test and I 2 statistics, using the forest plot.The calculated value of I 2 allows measuring the percentage of variability due to heterogeneity rather than chance difference or sampling error.If the value of I 2 was greater than 60% and the Q test yields P < 0.05, heterogeneity was considered statistically significant.The random effects model, based on the DerSimonian-Laird method, which calculates the variability within and between studies, was applied to estimate the pooled prevalence of the various variables (case type and gender) and 95% CIs.

Table 1 .
States with at least one reported case of Mpox in Nigeria.

Table 2 .
Risk of bias assessment for the Mpox systematic review.

Table 3 .
Studies included for analysis after full text evaluation, Mpox systematic review, Nigeria, 2022.NI not indicated.

Table 4 .
Mpox cases reported by gender, age and case definitions and outcome in publications under review.S.

no. Author and year of publication Gender Age Case definition Outcome Male Female Age range Average (in years) Suspected Confirmed
Figure 3. (A) Forest plot of the meta-analysis of suspected and confirmed cases.(B)Forest plot of the metaanalysis of confirmed cases and case fatality.(C)Forest plot of the meta-analysis of cases based on gender.Vol.:(0123456789)Scientific Reports | (2024) 14:7368 | https://doi.org/10.1038/s41598-024-58147-ywww.nature.com/scientificreports/(CI: 1.24-2.14 main search strategy used was: (("Monkeypox epidemiology"[All Fields] OR (("monkeypox"[MeSH Terms] OR "monkeypox"[All Fields]) AND ("epidemiology"[MeSH Subheading] OR "epidemiology"[All Fields] OR "surveillance"[All Fields] OR "epidemiology"[MeSH Terms] OR "surveillance"[All Fields] OR "surveillances"[All from World Health Organization, WHO; nine(9)from Nigeria Centre for Disease Control, NCDC; and two (2) from the European Centre for Disease Prevention and Control, ECDC.Others included two (2) from Nigeria Scholars; one (1) each from the websites of Nigeria Population Commission, NPC; Nigeria HIV/AID Indicator & Impact Survey, NAIIS; UNICEF; CDC; and National Action Plan for Health Security, NAPHS.These grey literature were included based on the relevance of their information to the topic under review.